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. 2014 Oct 14;23(1):63–70. doi: 10.1038/mt.2014.173

Figure 2.

Figure 2

In vitro competitive proliferation kinetics of mPB CD34+ cells transduced with VSV-LV and CD133-LV. (a) Lentiviral vectors encoding green or blue fluorescent proteins were packaged using VSV-G or CD133-targeting envelopes, respectively. Subsequently freshly isolated mPB CD34+ cells were transduced using both vectors and cultivated for up to 17 days in vitro in cytokine-supplemented media. (b) The fraction of transduced cells was analyzed in regular intervals by flow cytometry. (c) The average fold change of percentage transduced cells from six independent experiments is shown. Error bars: SD, P ≤ 0.001 at days 7 and 14.