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. Author manuscript; available in PMC: 2016 Apr 3.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Feb 26;459(2):259–263. doi: 10.1016/j.bbrc.2015.02.095

Fig. 3.

Fig. 3

Antibody interference with the autoprocessing of TcdA. TcdA was incubated with different antibodies and their mixture at 37°C for 1 hour in autoprocessing reaction buffer containing 25 μM InsP6. The reaction was terminated by SDS-sampling buffer and heated at 95 °C for 5 min. Western blot was performed for detecting cleaved GTD fragment from TcdA using a GTD-specific VHH antibody AH3.