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. Author manuscript; available in PMC: 2015 May 11.
Published in final edited form as: Mol Imaging. 2011 Dec;10(6):420–433.

Figure 6.

Figure 6

Determination of the intra-cellular distribution of SW116 in MDA-MB-435 cells with and without MitoTracker (A), ER-Tracker (B), LysoTracker (C), or a membrane tracker, FM1-43FX (D), using two-photon microscopy. MDA-MB-435 cells were incubated with 100 nM SW116 and either 20 nM Mito-Tracker, 500 nM ER-Tracker, or 100 nM LysoTracker for 2 hours at 37°C. MDA-MB-435 cells were also incubated with 100 nM SW116 and 5 μg/mL of the membrane tracker, FM1-43FX, for 15 minutes at 0°C. The live cells were imaged by two-photon microscopy.