Table 2.

Low-resolution targeted mass spectrometry measurements of lysine acetylated residues on CheY^a with and without acetate.

Peptide sequence	Acetylation site on CheY	Acetylated peptides [pM]
Experiment I		Absence of acetate	Presence of acetate
NLLacKELGFNNVEEAEDGVDALNK	K26	28.9	68.2
TIRADGAMSALPVLMVTAEAacK	K91	0	52.2
ADGAMSALPVLMVTAEAacKacK	K91, K92	0.03	0.52
LNacKIFEK	K122	0.4	13.35
Experiment II
NLLacKELGFNNVEEAEDGVDALNK	K26	5.4	6.4
TIRADGAMSALPVLMVTAEAacK	K91	0	137.05
LNacKIFEK	K122	0.42	13.35
Experiment III
IVRNLLacK	K26	3.2	1.7
NLLacKELGFNNVEEAEDGVDALNK	K26	2.5	0.3
TIRADGAMSALPVLMVTAEAacK	K91	142.2	322.3
LNacKIFEK	K122	5942.3	2793.3
LNKIFEacK	K126	3940.2	4692.3
LNacKIFEacK	K122; K126	128.5	261.3

^aIPTG-induced CheY.