Table 2.
Peptide sequence | Acetylation site on CheY |
Acetylated peptides [pM] | |
---|---|---|---|
Experiment I |
Absence of acetate |
Presence of acetate |
|
NLLacKELGFNNVEEAEDGVDALNK | K26 | 28.9 | 68.2 |
TIRADGAMSALPVLMVTAEAacK | K91 | 0 | 52.2 |
ADGAMSALPVLMVTAEAacKacK | K91, K92 | 0.03 | 0.52 |
LNacKIFEK | K122 | 0.4 | 13.35 |
Experiment II | |||
NLLacKELGFNNVEEAEDGVDALNK | K26 | 5.4 | 6.4 |
TIRADGAMSALPVLMVTAEAacK | K91 | 0 | 137.05 |
LNacKIFEK | K122 | 0.42 | 13.35 |
Experiment III | |||
IVRNLLacK | K26 | 3.2 | 1.7 |
NLLacKELGFNNVEEAEDGVDALNK | K26 | 2.5 | 0.3 |
TIRADGAMSALPVLMVTAEAacK | K91 | 142.2 | 322.3 |
LNacKIFEK | K122 | 5942.3 | 2793.3 |
LNKIFEacK | K126 | 3940.2 | 4692.3 |
LNacKIFEacK | K122; K126 | 128.5 | 261.3 |
IPTG-induced CheY.