Fig. 2.

Low-dose γ-radiation effects on adult climbing activity and longevity after irradiating Drosophila larvae. The rapid iterative negative geotaxis (RING) assay was used to measure fly locomotor activity. Climbing activity of (A) male and (B) female flies irradiated with 0.1 Gy γ-radiation was examined for flies aged 1 − 6 weeks and compared with that of control flies. Error bars represent SEM. Statistical significance was calculated using Student's t-test (*P < 0.05, **P < 0.01). Survival rates of control and irradiated (exposed to 0.1 Gy at the larval stage) flies were recorded. Age was measured in days after emergence. LDR had no effect on (C) male life span (P = 0.1592 with log rank test) or (D) female life span (P = 0.5750 with log rank test). Longevity analysis was performed three times, and data were analyzed with the Wilcoxon test.