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. 2015 May 7;9:15–25. doi: 10.4137/JEN.S22969

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© 2015 the author(s), publisher and licensee Libertas Academica Limited

This is an open-access article distributed under the terms of the Creative Commons CC-BY-NC 3.0 License.

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Overexpressed strumpellin and spartin colocalize. (A) Expressed N-tagged HA:strumpellin with the same subcellular localization as endogenous strumpellin in transfected cultured rat neurons. (B) Colocalization of WT overexpressed strumpellin with the early endosome antigen 1 (arrow). (C) (upper row) Coexpression of WT strumpellin:eGFP and full length (FL) spartin:myc in cos-7 cells; (middle row) coexpression of N471D strumpellin and FL spartin; and (bottom row) V629F strumpellin and FL spartin. Scale bar = 10 μm