Figure 6.

Knock-down of spartin did not alter endogenous strumpellin expression. (A) Western blotting of spartin after four different constructs targeting rat spartin were tested (lanes 1–4 correspond to four anti-spartin constructs and the fifth lane to a control, nonsensical RNA). (B) Rat cortical neurons expressing shRNA targeting spartin (construct #3) without any changes in endogenous strumpellin levels. (C) Plot of the expression levels of strumpellin, spartin, and WASH1 proteins normalized to actin levels (shown as value 1 on y-axis). (D) Semiquantitative analysis of neuronal branching assessed after 2 and 14 days. The y-axis shows the sum of counted primary and secondary branching, and the observed difference was not statistically significant. Scale bar = 5 μm.

Note: *p < 0.001.