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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Aug 21;54:1–10. doi: 10.1016/j.ibmb.2014.08.001

Figure 6.

Figure 6

Insulin signaling pathway controls expression of the CP-Gal4>UAS-EGFP transgene. (A) RNAi of Insulin Receptor (dsInR) in vivo. RNAi for Luciferase (dsLuc) served as a control. The CP-Gal4>UAS-EGFP females were injected with dsRNAs at 1 day post-eclosion, blood fed and examined at 24 h PBM. Transcript abundance was examined using Gal4 and EGFP primers by means of qRT-PCR. Control dsLuc transcript level was standardized to 1, while transcript levels for other RNAi treatments were expressed relative to the control. (B–C). Activation of the CP-Gal4>UAS-EGFP transgene expression by insulin in vitro was prevented by dsInR. The CP-Gal4>UAS-EGFP females were injected with dsRNAs at 1-day post-eclosion, and an in vitro assay was performed as in Figure 5. Midguts were isolated from non-blood fed CP-Gal4>UAS-EGFP females and incubated in the AA-containing culture medium. Expression of the CP-Gal4>UAS-EGFP transgene was monitored by means of qRT-PCR using probes for Gal4 (B) and EGFP (C) (primer sequences in Table S3).