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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Aug 21;54:1–10. doi: 10.1016/j.ibmb.2014.08.001

Figure 7.

Figure 7

Effect of TOR inhibitor rapamycin on the expression of the CP-Gal4>UAS-EGFP transgene. (A). In vitro application of AAs and insulin activated CP-Gal4>UAS-EGFP expression, and rapamycin was capable of blocking this activation. The transcript level was set at 1 for the “no treatment” sample (control), and transcript levels for the other treatments were expressed relative to the control. (B). qRT-PCR analysis of CP-Gal4>UAS-EGFP transgene expression in midguts at 24 h PBM from CP-Gal4>UAS-EGFP hybrid females after different treatments. 20 pmol of rapamycin was injected in each experiment. Transcript levels were standardized to 1 for the dsLuc sample (control), and transcript levels for the other treatment were expressed relative to the control.