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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Insect Biochem Mol Biol. 2014 Aug 21;54:1–10. doi: 10.1016/j.ibmb.2014.08.001

Figure 8.

Figure 8

RNAi of the EcR (dsEcR) and Br-Z4 (dsBr-Z4) gene in the CP-Gal4>UAS-EGFP female mosquitoes. (A–C). The procedure was performed as in Figure 6A. RNAi for Luciferase (dsLuc) served as a control. The effect of the EcR and Br-Z4 knockdown on the CP-Gal4>UAS-EGFP transgene expression was monitored by means of qRT-PCR using Gal4 and EGFP primers. The transcript levels of the endogenous CP gene (A) and Br-Z4 gene (B) after dsEcR treatment were also examined. Times at which examinations were done are marked by 24h and 36h PMB.