Table I. Enumeration of memory P14 CD8 T cells by cell isolation and flow cytometry and QIM.
5×104 naїve Thy1.1+ P14 CD8 T cells were transferred to C57BI/6J mice, which were infected one day later with 2×105 LCMV Armstrong i.p. 120-150 days later, 3min prior to sacrifice mice were injected i.v. with α-CD8α antibody to discriminate the blood and marginated pool (i.v.+) from parenchymal P14 (i.v.-). Bold values indicate the average number of P14 or total nucleated cells per tissue derived from cell isolation and flow cytometry or QIM. Kidney accounts for both kidneys, salivary gland reports for both lobes, uterus includes both uterine horns, and mandibular lymph node enumerates a Single unpaired lymph node. Peripheral blood enumeration is extrapolated to 1.74mL of blood, based on average body weight of mice used in this study. Data from 6 or more mice. Underlined italic numbers indicate number of nucleated cells (± SD) as determined by DNA extraction. BD= below detection, NA= not available, LP= lamina propria, IEL= intraepithelial lymphocytes, M.E.= muscularis externa, ILF= isolated lymphoid follicle.
| Tissue | Fold Difference | Flow Cytometry | QIM | QIM & DNA | |||
|---|---|---|---|---|---|---|---|
| QIM/Flow | Total P14 ×104±SD ×104 | % of total P14 i.v.- | Total P14 ×104±SD ×104 | % of total P14 i.v.- | P14 ×103±SD ×103 Per 106 nuclei | Total Nucleated Cells ×106±SD×106 | |
| Spleen (White Pulp) | 1.92 | 195 ±79.7 | 66.6 | 375 ±142 | 82.1 | 25.7 ±9.41 | 178 ±10.7237 ±30.2 |
| Spleen i.v.+ (Red Pulp) | 0.84 | 93.2 ±32.7 | 78.5 ±20.5 | ||||
| Mandibular lymph node | 1.19 | 5.31 ±2.00 | 97.7 | 6.32 ±2.83 | BD | 20.7 ±7.74 | 2.98 ±0.4811.5 ±0.705 |
| Thymus | 9.26 | 1.96 ±1.11 | 96.6 | 99.7% IV-= 18.1 ±5.61 | 99.7 | 2.86 ±0.32 | 77.3 ±25.7 |
| Liver | 6.13 | 6.12 ±2.05 | 14.8 | 37.5 ±22.0 | 16.9 | 5.42 ±1.17 | 378 ±29.91180±973 |
| Liver i.v.+ | 4.58 | 37.1 ±75.9 | 170 ±36.1 | ||||
| Lung | 69.1 | 0.31 ±0.24 | 9.06 | 21.3±15.3 | 25 | 3.36 ±1.27 | 282 ±45.2 |
| Lung i.v.+ | 13.8 | 5.43 ±6.42 | 75.2±53.7 | ||||
| Kidney | 27.2 | 0.945 ±0.64 | 46.3 | 25.4 ±8.27 | 83.1 | 1.99 ±0.697 | 157 ±25.0 |
| Kidney i.v.+ | 5.02 | 1.03 ±0.76 | 5.17 ±1.95 | ||||
| Pancreas | 13.3 | 2.87 ±2.11 | 94.8 | 99.7% IV-= 37.9 ±9.19 | 99.7 | 4.37 ±0.95 | 86.9 ±11.5 |
| Salivary (serous) | 13.1 | 1.65 ±0.59 | 99.8 | 21.6 ±5.61 | BD | 10.3 ±2.64 | 21.2 ±2.58 |
| Salivary (mucous) | NA | NA | NA | 7.81 ±2.35 | BD | 5.43 ±1.41 | 14.4 ±1.42 |
| Uterus | 69.0 | 0.603 ±0.41 | 90.2 | 25.6 ±4.61 | BD | 3.51 ±0.958 | 75.7 ±15.885.4 ±26.2 |
| Cervix/Vagina | 16.0 ±8.39 | BD | 2.95 ±1.02 | 52.4 ±14.245.1 ±19.8 | |||
| SI (IEL) | 6.10 | 3.81 ±2.02 | 99.8 | 23.2 ±12.7 | BD | 3.24 ±0.91 | 328 ±92.9517 ±176 |
| SI (LP) | 18.6 | 4.06 ±1.70 | 99.1 | 74.3 ± 22.5 | BD | ||
| SI (muscle) | 1.04 ±1.05 | BD | |||||
| LI (IEL) | 41.3 | 0.034 ±0.018 | 84.10 | 1.39 ±0.75 | BD | 0.81 ±0.04 | 122 ±12.3 |
| LI (LP) | 68.1 | 0.12 ±0.073 | 82.46 | 7.59 ±3.65 | BD | ||
| LI (ILF) | 0.51 ±0.63 | BD | |||||
| Stomach(IEL) | 17.5 | 0.35E ±0.45 | 91.9 | 6.17 ±2.27 | BD | 2.91 ±0.92 | 118 ±14.9113 ±17.4 |
| Stomach (LP) | 122 | 0.22 ±0.157 | 95.1 | 20.0 ±7.22 | BD | ||
| Stomach (Submucosa) Stomach(M.E) | 3.05 ± 0.92 | BD | |||||
| 3.91 ±1.12 | BD | ||||||
| Peripheral Blood | NA | 13.4 ±6.33 | 100 | NA | NA | NA | NA |