Figure 6. Additive effect on the activation energy for fusion induced by PDBu causes supralinear effect on release kinetics.
(A) Current traces, (B) release rate constants k2,max, and (C) activation energies for fusion at different sucrose concentrations in the absence and presence of PDBu. PDBu-induced changes in k2,max and ΔEa, obtained by subtraction of the data curves in B and C before and after PDBu application, show (D) an exponential increase in k2,max for increasing sucrose concentrations whereas (E) the changes in the energy domain are in the same order of magnitude (reduction at 0M is probably an overestimation due to Ca2+ depenence of the spontaneous release, [see text]). Mean values of k2,max displayed are all within the 95% confidence interval as determined by Bootstrap analysis.
DOI: http://dx.doi.org/10.7554/eLife.05531.022
Figure 6—source data 1. Parameter values for Figure
6B–E, bootstrap analysis Figure 6, Figure 6—figure supplement 3A–D, and
Figure 6—figure
supplement 3.
elife05531s006.docx (33.6KB, docx)
DOI: 10.7554/eLife.05531.023
Figure 6—figure supplement 1. Random examples of individual HS-evoked EPSCs (black) in the absence of PDBu, overlaid with their best fit (red).
(A) Responses to 0.5M. (B) Responses to
0.3M.
Figure 6—figure supplement 2. Random examples of individual HS-evoked EPSCs (blue) in the presence of PDBu, overlaid with their best fit (red).
(A) Responses to 0.5M. (B) Responses to
0.3M.
Figure 6—figure supplement 3. Upstream parameters and RRP size are not affected by PDBu application.
(A) Priming rate
k1D. (B)
Unpriming rate constant k−1.
(C) RRP size. (D) Relation between
k2,max and depleted RRP
is maintained in the presence of PDBu, but synaptic responses to
submaximal HS-stimulation display faster kinetics and more RRP
depletion.