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. 2015 Apr 14;4:e05531. doi: 10.7554/eLife.05531

Figure 7. Additive effect on the activation energy for fusion induced by Cpx deletion causes supralinear effect on release kinetics.

(A) Current traces, (B) release rate constants k2,max, and (C) fusion energy barrier heights at different sucrose concentrations for control and CpxKO cells. Cpx deletion-induced changes in k2,max and ΔEa, obtained by subtraction of the data curves for control and CpxKO in B and C, show (D) an exponential increase in k2,max for increasing sucrose concentrations whereas (E) the changes in the energy domain are in the same order of magnitude. Mean values of k2,max displayed are all within the 95% confidence interval as determined by Bootstrap analysis. Cpx data were published before in (Xue et al., 2010) and reanalysed here.

DOI: http://dx.doi.org/10.7554/eLife.05531.027

Figure 7—source data 1. Parameter values for, bootstrap analysis Figure 7B–E, bootstrap analysis Figure 7, Figure 7—figure supplement 1A–D, and bootstrap analysis Figure 7—figure supplement 1.
DOI: http://dx.doi.org/10.7554/eLife.05531.028
elife05531s007.docx (31.3KB, docx)
DOI: 10.7554/eLife.05531.028

Figure 7.

Figure 7—figure supplement 1. Upstream parameters and RRP size are not affected in Cpx KO.

Figure 7—figure supplement 1.

(A) Priming rate k1D. (B) Unpriming rate constant k−1. (C) RRP size. (D) Relation between k2,max and depleted RRP is maintained in Cpx KO synapses, but synaptic responses to submaximal HS-stimulation display slower kinetics and less RRP depletion.
DOI: http://dx.doi.org/10.7554/eLife.05531.029