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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biotechnol Bioeng. 2015 Feb 20;112(4):838–842. doi: 10.1002/bit.25486

Figure 2.

Figure 2

Effect of shear stress on IL-1β secretion from C. pneumoniae-infected monocytes. Human monocytes were infected with mock (media only) or C. pneumoniae EB (MOI 1) for 2.5 h. 48 h post infection, uninfected and infected cells at a concentration of 6×106 cells/ml were sheared for 1 hour at 0 (static) and 5 dyn/cm2 (shear) using a cone-and-plate viscometer. After shear exposure, the cells were incubated for further 0 or 4 h, and supernatants and cell lysates were analyzed for IL-1β by ELISA. The results are expressed as actual levels (A) and fold increase (B) from one representative experiment performed for four donors and statistical difference was evaluated using two-way ANOVA (*p<0.05).