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. Author manuscript; available in PMC: 2015 May 11.
Published in final edited form as: Gastroenterology. 2013 Jul 12;145(4):842–52.e2. doi: 10.1053/j.gastro.2013.07.001

Figure 6.

Figure 6

DNA-methylation regulates miR-124 expression in active pediatric-UC. (A) Levels of DNA-methylation of MIR124 promoter region in active pediatric-UC biopsies (pUC), compared to controls (pControl) were determined by bisulfate sequencing. Values represent mean ±SD; t-test, Prism6 (GraphPad Software, Inc.). (B) Correlation of miR-124 expression levels with the DNA-methylation status of the MIR124 promoter. Correlation coefficient was calculated using Prism6 (GraphPad Software, Inc.). (C) Real-time PCR analysis for the expression levels of miR-124 and (D) STAT3, after treatment of HCT-116 colonic cells with two different concentrations (1 and 5 µM) of the DNA-methyltransferase inhibitor 5-aza-2’-deoxycytidine (5-AZA), for 0 to 4 days. The experiment was performed in triplicates.