Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Method | Sitting-drop vapor diffusion |
| Plate type | Greiner Bio-One 96-well CrystalQuick standard profile, 3 round wells, flat bottom |
| Temperature (K) | 289 |
| Protein concentration (mgml1) | 13.4 |
| Buffer composition of protein solution | 20mM HEPES pH 8.0, 150mM KCl, 1.5mM TCEP |
| Composition of reservoir solution | 0.1M BicineNaOH pH 9.0, 20% PEG 6000 |
| Cryocondition | 0.1M BicineNaOH pH 9.0, 20% PEG 6000, 15% glycerol |
| Volume and ratio of drop | 1:1 (0.4:0.4l) |
| Volume of reservoir (l) | 135 |
