Table 1. Crystallization.
| Sample | PDB entry | Protein concentation (mgml1) | Cofactor concentration (mM) | Crystallization screen condition | Reservoir composition |
|---|---|---|---|---|---|
| TrcrA.00019.a.B1, apo | 4fzi | 37 | Morpheus H1 | 0.1M MESimidazole pH 6.5, 10% PEG 20000, 20% PEG 550 MME, 0.02M glutamic acid, glycine, serine, alanine and lysine | |
| TrcrA.00019.a.B1 + NADP | 4gie | 37 | 4 | Index G6 | 0.2M ammonium acetate, 0.1M bis-tris pH 5.5, 25% PEG 3350; cryoprotectant 15% ethylene glycol |
| LemaA.00019.a.B1, apo | 4f40 | 25 | JCSG+ A2 | 0.1M sodium citrate pH 5.50, 20% PEG 3000 | |
| LemaA.00019.a.B1 + NADPH | 4g5d | 25 | 2 | JCSG+ B8 | 200mM magnesium chloride, 100mM Tris pH 7.0, 10% PEG 8000 |