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. 2015 May 11;10(5):e0127179. doi: 10.1371/journal.pone.0127179

Fig 2. Generation of transgenic banana plants overexpressing MusamirRNA156.

Fig 2

(A) Schematic representation of the T-DNA region of the binary vector p1301-MusamiRNA156 depicting the insertion of the stem-loop sequence of MusamirRNA156 primary transcript in the multiple cloning site of modified pCAMBIA-1301 vector downstream of Zea mays polyubiquitin promoter. Putatively transformed embryos selected on the medium supplemented with hygromycin (B) followed by germination. The shoots were multiplied in medium containing BAP to form the clonal copies of the shoots (C) which were further rooted in NAA based medium (D).