Skip to main content
. 2015 May 11;10(5):e0127179. doi: 10.1371/journal.pone.0127179

Fig 3. Expression analysis of the T-DNA genes in transgenic banana lines.

Fig 3

(A) The expression of the GUS cassette in transgenic banana lines was determined by histochemical staining of the leaf discs and showed the presence of intense blue coloration in the transgenic lines (OV) whereas no colour was observed in the untransformed control plants (UC). (B) The quantum of the primary transcript of MusamirRNA156 in the transgenic banana line and untransformed control plant was determined by RT-PCR wherein a 175 bp product was seen in both untransformed control plant and selected transgenic line with later being brighter. (M- 1kb DNA ladder; N- RT-PCR control)