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. 2015 Apr 25;71(Pt 5):1184–1196. doi: 10.1107/S1399004715004514

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© Coquelle et al. 2015

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Specific radiation damage is observed in both the micro and the nano data sets, but does not compromise the structural information. (a) Structure-factor amplitude Fourier difference (F _o − F _o) maps were calculated between our data sets (conservative micro and conservative nano) and four other HEWL data sets: the SFX data set, the Flow-SSX data set and two low-dose data sets collected at either 100 or 295 K. The maps are displayed around the four disulfide bridges of lysozyme, with red and green contours indicating negative and positive density, respectively. (b) Unbiased 2mF _o − DF _c (blue; contour level 1.0 σ) and mF _o − DF _c (red and green; contour level ± 3σ) maps are displayed around the four disulfide bridges of lysozyme.