MLR of CD4+ T-enriched lymphocytes. a, Proliferative response of BALB/c CD4+ T cells to B6 control and B6 → C2D chimeric stimulators and lack of proliferation to C2D control and C2D → B6 chimeric stimulators (splenocytes). b, Proliferative response of BALB/c unseparated LN cells to B6, B6 → C2D, C2D, and C2D → B6 stimulator splenocytes. Note the typical proliferative response to C2D control and C2D → B6 chimeric splenocytes, reflecting proliferation of CD8+ responders. Data are representative of three experiments testing CD4 T cell-dependent reactivity to donor splenocytes derived from chimeric animals.