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. 2015 May 11;10(5):e0126968. doi: 10.1371/journal.pone.0126968

Fig 4. Hypoxia downmodulates TM9SF4 expression in leukemic cells.

Fig 4

(A, B) The decrease of TM9SF4 protein expression in U937 cells (A) and in primary leukemic cells from AML M1 (B) cultured under hypoxic (1% and 5% O2, respectively) compared to normoxic (21% O2) conditions is shown by immunofluorescence analysis of TM9SF4. ctr- is for no primary antibody negative control. DAPI shows nuclear staining; AlexaFluor-647 shows TM9SF4 protein staining; DAPI+647 is the merge of AlexaFluor-647 and DAPI; Trasmitted shows the phase-contrast microscopy fields; Scale bars indicated are 10μm (A) and 30μm (B).