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. 2015 May 11;10(5):e0127196. doi: 10.1371/journal.pone.0127196

Fig 5. Life spans and telomere lengths in D. pulex (RW20) and D. pulicaria (LakeXVI-clone11).

Fig 5

A) Survivorship curves of clones RW20 (open squares) and XVI-11 (black circles). Error bars show age-specific standard errors from Kaplan-Meier survival probability estimates. B) Terminal Restriction Fragment (TRF) assay was performed to estimate the average telomere length of the Daphnia at various ages. The age in weeks is indicated above each lane. Lanes 1–3: D. pulex, lanes 4–6: D. pulicaria. M1 and M2: molecular weight markers; lambda HindIII digest and lambda BstEII digest respectively. C) Quantification of 5 A, error bars indicate standard deviation. Student T-tests were performed and p values are as follows: * = 1.03x10-5, ** = 0.007, *** = 2.48x10-5 (n = 3).