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. 2015 May 11;10(5):e0126607. doi: 10.1371/journal.pone.0126607

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© 2015 Huan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — A, the morphologies of 35-day fetuses, B and C, the corresponding methylation statuses of H19/Igf2 in 35-day fetuses. IV, the in vivo produced fetuses, IVF, the IVF fetuses, NT-CON-N, the normal cloned fetuses in the NT-CON group, NT-CON-A, the abnormal cloned fetuses in the NT-CON group, NT-TSA-N, the normal cloned fetuses in the NT-TSA group, and NT-TSA-A, the abnormal cloned fetuses in the NT-TSA group. TSA prevented the H19/Igf2 imprinting disruption and morphological abnormality of cloned fetuses. Black or white circles indicate methylated or unmethylated CpG sites. The data were expressed as mean ± SEM. ^a-cValues with different superscripts differ significantly (P<0.05).