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. 2015 May 11;10(5):e0125627. doi: 10.1371/journal.pone.0125627

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© 2015 Devi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — Paraffin embedded sections of human term placenta (A-F) and frozen sections of idiopathic preterm placenta (G-I) with some attached fetal membrane were analyzed for IL-6 or IGFBP-1 expression by immunohistochemistry. Decidual cells were identified by morphological analysis or IGFBP-1 staining. (A) Term placenta/fetal membrane, control immunostaining. (B) Term placenta/fetal membrane, IL-6 expression is shown in red staining, decidual cells (arrows). (C) Term placenta/fetal membrane, IGFBP-1 expression is shown in brown staining. A-C: adjacent slides of same placenta/fetal membrane. (D) Term placenta/fetal membrane, control immunostaining. (E) Term placenta/fetal membrane, IL-6 expression is shown in red staining, decidual cells (arrows), trophoblast cells (yellow arrows). (F) Term placenta/fetal membrane, IGFBP-1 expression is shown in brown staining. D-F are adjacent slides of another term placenta/fetal membrane at higher magnification. (G) Preterm placenta/fetal membrane, control immunostaining. (H) Preterm placenta/fetal membrane, IL-6 expression is shown in red staining, decidual cells (arrows), trophoblast cells (yellow arrow). (I) Preterm placenta/fetal membrane, IGFBP-1 expression is shown in brown staining. G-I are adjacent slides of idiopathic spontaneous preterm placenta/fetal membrane. At least 3 different placentas were used in each group. Shown in the figure are representative tissue slides. Original magnification: A-C, 20X; D-I, 40X.