Fig 3. Dose dependent inhibition of IL-1β-stimulated IL-6 expression by curcumin.

HuF cells were treated with vehicle (control), IL-1β (10ng/ml), or with combinations of IL-1β (10ng/ml) and different doses of curcumin (0–40μM) for 24 hr. Total mRNA was analyzed for IL-6 expression by qPCR and RT- PCR. RPS17 expression was used as control. The values are expressed as the means ± S.E. (n = 3). ab, p < 0.001; bc, p < 0.001; ac, p < 0.01.