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. Author manuscript; available in PMC: 2015 May 11.
Published in final edited form as: Nat Med. 2015 Mar 16;21(4):389–394. doi: 10.1038/nm.3819

Figure 4.

Figure 4

Mtus1 and Kcnk3 are required for beige adipocyte differentiation and thermogenic function. (a) Expression of Mtus1, Kcnk3, and Fzd8 in mouse inguinal WAT-derived adipocytes transfected with siRNAs targeting the indicated genes or non-targeting control (Ctrl). n = 4 for all groups. ***P < 0.001 for indicated siRNA versus control by one-sided Welch’s t-test. (b) Ucp1, Mtus1 and Kcnk3 protein expression from cells under the same culture condition as in a. β-actin, loading control. (c) Expression of Ucp1, Cidea, Cox7a1, and Adipoq (encoding adiponectin) from cells under the same culture condition as in a. n = 4 for all groups. **P < 0.01, ***P < 0.001 indicated siRNA versus control by one-sided Student’s t-test. NS, not significant. (d) Total and uncoupled cellular respiration. n = 8 for all groups. **P < 0.01, ***P < 0.001 by one-sided Welch’s t-test. Data are expressed as means ± s.e.m. for all bar graphs. Data are representative of three independent experiments.