Figure 4.
Increased expression of M1 and M2 macrophage markers in the adipose tissue of infected mice. Relative levels of arginase (Arg1), nitric oxide synthase 2, inducible (Nos2), interferon-γ (Ifng) and interleukin 10 (Il10) mRNA, normalized to Non-POU-domain containing octamer binding protein (Nono) mRNA, detected by real-time PCR in the stromal vascular fraction (SFV) of gonadal adipose tissue (GAT) of mice 7 days after intraperitoneal administration of 1 × 107 Neospora caninum tachyzoites (NcT) or PBS (PBS). Each symbol represents an individual mouse. Horizontal lines represent the mean values of the respective group (± SD). These results are pooled from three independent experiments. (Mann–Whitney U-test, *P < 0·05; **P ≤ 0·01; ****P ≤ 0·0001).