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. 2015 May 7;145(2):258–267. doi: 10.1111/imm.12442

Figure 4.

Figure 4

Role of ATP release and the purinergic signalling pathway in enterohaemorrhagic Escherichia coli (EHEC) O157:H7 enterohaemolysin-induced interleukin-1β (IL-1β) production by THP-1 cells. Differentiated human THP-1 cells were infected with EDL933, ΔehxA or ΔehxA/pehxA. (a) ATP release from the differentiated THP-1 cells at 4 hr post-infection. (b) Positive correlation between ATP release and IL-1β production in the supernatant of THP-1 cells infected with EHEC O157:H7. (c) The differentiated THP-1 cells were treated with 500 nm of the ATP inhibitor oATP for 2 hr before infection. The IL-1β concentration in the supernatant was determined at 4 hr post-infection by ELISA. (a, c) The results represent the mean ± SD of three independent experiments. Significant differences (*P < 0·05, **P < 0·01) are indicated. n.s., not significant (P > 0·05).