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. 2015 May 7;145(2):258–267. doi: 10.1111/imm.12442

Figure 6.

Figure 6

The role of K+ efflux in enterohaemorrhagic Escherichia coli (EHEC) O157:H7 enterohaemolysin-induced interleukin-1β (IL-1β) production and cytotoxicity in THP-1 cells. Human THP-1 cells were infected with EHEC O157:H7 for 4 hr in the presence of 140 mm K+. (a) The IL-1β concentration in the supernatants was determined 4 hr post-infection by ELISA. (b) Cytotoxicity was evaluated by the release of lactate dehydrogenase (LDH) from the THP-1 cells, which was assayed after 4 hr of exposure to EHEC O157:H7. The results represent the mean ± SD of three independent experiments. Significant differences (**P < 0·01) are indicated. n.s., not significant (P > 0·05).