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. 2015 May 11;10(5):e0124033. doi: 10.1371/journal.pone.0124033

Fig 2. Sesn2 inhibits in vitro cellular transformation and in vivo tumor formation of lung epithelial cells.

Fig 2

(A) Knocked down of endogenous Sesn2 by two independent shRNAs targeting 3’ UTR of Sesn2 transcripts, was confirmed by immuno-blotting. (B) The average number of colonies formed in shSesn2-2 transformed cells were 4 times higher than shFF2 transformed cells, *p<0.05. Ectopic expression of Sesn2 re-suppressed the anchorage independent growth in the shSesn2-2 expressing cells. (C), (D) and (E) Knockdown of Sesn2 in BEAS-2B cells led to enhanced tumor formation in nude mice. The Sesn2 silenced cells were transplanted into the armpits of Balb/c nude mice, xenografts were formed one month later post transplantation and photographed, red arrows indicated formed tumors under the mouse skin (upper panel of C). The image of two representative dissected tumors was shown in the lower panel of C. The average tumor volumes and weights from transplanted mice with Sesn2 silencing and FF2 control cells were measured and plotted in D and E, data are presented as mean ± standard deviation per mouse group, *p<0.05 by the Student t-test.