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. 2015 May 11;10(5):e0124033. doi: 10.1371/journal.pone.0124033

Fig 3. Silence of Sesn2 promotes cell growth and cell migration.

Fig 3

(A) Conformation of Sesn2 knockdown in A549 cells by immuno-blotting. (B) The cell cycle profiles of lung adenocarcinoma A549cell lines expressing shSesn2-2 and shFF2. Cells were stained with propidium iodide and analyzed by flow cytometry. The higher S phage proportion detected in shSesn2-2 cells suggested faster cell cycle progression compared to shFF2 cells. (C) Representative images of wound healing experiment performed in shSesn2-2 and shFF2 expressing cells at the indicated times. The average wound closure percentages in each cell group were plotted in (D), significant healing difference were observed after 24 hour of wound healing, *p<0.05. Experiments were performed in triplicate. (E) The cell growth rates of shSesn2-2 and shFF2 expressing cells were determined by MTT assays and compared by the two-way Anova test (*p<0.05).