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. 2015 May 11;10(5):e0124033. doi: 10.1371/journal.pone.0124033

Fig 4. Down-regulation of Sesn2 by shRNA causes enhanced activation of Akt-mTOR-p70S6K signaling upon insulin stimulation.

Fig 4

(A) A549 cells stably expressing shSesn2-2 or shFF2 were serum-starved overnight and re-stimulated with 100nM insulin for 20 min, the expression levels of Akt, p70S6K and their phosphorylated species were detected by immuno-blotting with indicated antibodies. (B) The intensity of protein bands on the immuno-blots was quantified with densitometry scanning. The relative expression level of each protein was normalized based on the level of GAPDH in each lysate and plotted in B, data were shown as mean ± standard deviation (SD) from three independent experiments. *p<0.05 indicates a significant difference in the expression levels of the examined proteins in the two cell lines.