Fig 2. Expression of T3SS genes up-regulated by HrpG depends on the wild type copy of hrpG.

(A) qRT-PCR of hrpX, hrcC and hrpB2 of total RNA obtained from Xcc, deletion mutant ΔhrpG, ΔhrpG-R210C, ΔhrpG-HrpG, Xcc-R210C and Xcc-HrpG strains grown in XVM2 were assayed. (B) As in (A) but RNA was obtained from bacteria recovered from infiltrated tissue at 3 and 6 dpi before RNA extraction. As a reference gene, a fragment of rpoB gene was amplified. Values represent the means of four independent experiments. Error bars indicate standard deviations. Data were statistically analyzed using one-way ANOVA (p<0.05).