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. 2015 May 11;10(5):e0125516. doi: 10.1371/journal.pone.0125516

Fig 4. HrpG-R210C is unable to activate transcription from HrpX promoter.

Fig 4

RNA was generated in vitro from a PCR product template containing the promoter as well as 200 bp downstream of the putative transcription start site of the corresponding gene. For in vitro transcription, templates were incubated with 16 (lane 3) and 32 pmol (lane 4) of HrpG-R210C protein or 16 (lane 5) and 32 (lane 6) of HrpG protein before the start of transcription by addition of 1 U of RNAP and NTPs. Negative controls were set up without addition of RNAP (lane 1) or HrpG (lane 2).