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. 2015 May 11;10(5):e0125516. doi: 10.1371/journal.pone.0125516

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© 2015 Ficarra et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Pull-down assays showing in vitro interaction studies with HrpG fused to GST (GST-HrpG) and HrpG and HrpG-R210C fused to thioredoxin. Proteins eluted from the matrix were analyzed by immunoblotting using anti-His and anti-GST antibodies. (B) Western and Far-Western blots showing interactions between HrpG and HrpG and HrpG-R210C fused to thioredoxin. The Western blot was incubated with anti-GST (left panel) and in the Far-Western blot (right panel) the nitrocellulose membranes were overlayed with 50 μg of GST-HrpG and after washing, probed with anti-GST antibody.