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. 2015 May 11;10(5):e0125516. doi: 10.1371/journal.pone.0125516

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© 2015 Ficarra et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) HrpG and HrpG-R210C were subjected to trypsin proteolysis for 30 min at room temperature. Lanes 1 and 5 are the undigested samples (5 μg), lanes 2 and 6, proteins were digested with 1:250, lanes 3 and 7 with 1:125 and lanes 4 and 8 with 1:62.5 (w/w) trypsin. (B) A mixture 1:1 of HrpG and HrpG-R210C was subjected to trypsin proteolysis for 30 min at room temperature. Lanes 1 is the undigested sample (5 μg), in lanes 2–4 proteins were digested with 1:250, 1:125 and 1:62.5 (w/w) trypsin, respectively.