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. 2015 May 11;10(5):e0126499. doi: 10.1371/journal.pone.0126499

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© 2015 Ma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) A schematic representation showing the putative target site of Bag-1 and mutated target site for miR-138 with the seed region and base substitutions underlined. (B and C) Luciferase reporter assay in HEK293T cells with co-transfection of Bag-1-UTR-WT or Bag-1-UTR-MUT at indicated times. (D and E) The mRNA and protein expression levels of Bag-1 in OCUG-1 and NOZ cells transfected with miR-138 mimic or the control. β-Actin was used as an internal quantitative control. Error bars represent the SD from three independent trials. **P<0.01.