Figure 12.

In vitro effects of metformin. (A) Cultured human foreskin fibroblast cell lines were incubated with four different concentrations of metformin (0–50 μg/L). Half of the plates were pre-treated with metformin for 8 h before adding the parasites. In the other half, metformin was added to the plates at the same time parasites were added. Each bar represents the average number of live trypomastigotes in the culture medium (determined by haemocytometer) at 24 h post-inoculum. (B) With metformin at 0 and 10 μg/L, parasite counts were determined after 24, 48 and 72 h. Each bar represents the average number of live trypomastigotes in the medium at each time point after innoculum.