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. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Mol Cell. 2015 Apr 16;58(3):431–439. doi: 10.1016/j.molcel.2015.03.019

Figure 6.

Figure 6

UV stress attenuates REP-based translational repression of NrdA by elimination of trans-translation. (A) The amount of NrdA protein and nrdAB mRNA in strains with either 9 nt (S9) or 40 nt spacing (S40) between the nrdA stop codon and its downstream REP sequence. Cells were untreated or exposed to UV treatment as described in “Experimental Procedures”. NrdA was detected with FLAG antibody, nrdAB with oligo N8. (B) Amount of total NrdA and tmRNA (DD) tagged NrdA protein in wild type (AA) and tmRNA mutant (DD) strains containing 9 nt between the nrdA stop codon and its downstream REP sequence with or without UV treatment. NrdA (DD) was detected with tmRNA (DD) antibody as described in “Experimental Procedures”. Cells were treated with UV as described in “Experimental Procedures” and the amount of NrdA protein and nrdAB message was determined by Western or Northern analysis as in panel A. Either 40 μg of total RNA or 5 μg of total protein were added to each lane.