Skip to main content
. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Cell. 2015 Apr 30;161(4):868–878. doi: 10.1016/j.cell.2015.04.005

Figure 3. Deletion of nmad-1 accelerates the progressive fertility defect of spr-5 mutant worms.

Figure 3

A) Phylogeny tree of human and C. elegans ALKBH family members. B) Knockdown of 4 of the ALKBH family members has no effect on egg laying of WT and spr-5 mutant worms treated for 20 generations with bacteria expressing the specific dsRNAs. Knockdown efficiency was tested by real-time RT PCR (Figure S6A). C) Early generation (G5) spr-5 mutant worms do not display significant fertility defects, but when combined with nmad-1 deletion these worms become sterile by generation 4. Each bar represents the mean ± SEM of 3 independent experiments. D) nmad-1 mutants lay fewer eggs than WT worms but do not display a progressive fertility decline. Each bar represents the mean ± SEM of 2–6 independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns not significant.