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. Author manuscript; available in PMC: 2016 May 7.
Published in final edited form as: Mol Cell. 2015 Apr 16;58(3):541–548. doi: 10.1016/j.molcel.2015.03.014

Figure 3.

Figure 3

RIG-IΔN relieves the viral suppression of the MDA5 signaling activity.

A. Impact of RIG-IΔN or MDA5ΔN on the IFNβ reporter activity of full-length MDA5 or RIG-I in the absence of viral proteins. Wild-type (WT) and their catalytic mutants, K270A (for RIG-I) and K335A (for MDA5), were compared. Data are shown in mean ± SD (n=3).

B. Impact of full-length NS1, E3L and their RNA-binding defective mutants on the signaling activities of MDA5, RIG-I and MAVS. See Figure S3E for western blot analysis.

C. Impact of RIG-IΔN or MDA5ΔN on the IFNβ reporter activity of full-length MDA5 in the presence of NS1 or E3L. Data are shown in mean ± SD (n=3).

D. Model in which RIG-I indirectly promotes the MDA5 signaling activity by displacing NS1/E3L from dsRNA.

E. Impact of RIG-IΔN or MDA5ΔN on the IFNβ reporter activity of full-length RIG-I in the presence of NS1 or E3L. Data are shown in mean ± SD (n=3).

F. Model in which the RIG-I signaling activity is affected by NS1/E3L and MDA5ΔN. NS1/E3L suppress RIG-I through both RNA-dependent and -independent mechanisms. Accordingly, the inhibitory effect of NS1/E3L on RIG-I could not be relieved by the protein displacement activity of MDA5.