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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Muscle Res Cell Motil. 2015 Jan 23;36(2):169–181. doi: 10.1007/s10974-015-9404-6

Figure 2. MS/MS characterization of the mono-phosphorylated sTnT(III).

Figure 2

Fragment assignments were made to the DNA-predicted sequence of human ssTnT (UnitprotKB/Swiss-Prot P13805-3) with the removal of N-terminal Met, acetylation and phosphorylation at the new N-terminus. A total of eight sets of MS/MS data including five CAD and three ECD spectra were analyzed, and a total of 181 out of 260 bond cleavages are shown. Based on the fragmentation map, the most abundant peak with a molecular mass of 31232.20 Da was confirmed to be the mono-phosphorylated peak of ssTnT isoform III (P13805-3) with the phosphorylation site at the N-terminus.