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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Muscle Res Cell Motil. 2015 Jan 23;36(2):169–181. doi: 10.1007/s10974-015-9404-6

Figure 4. High-resolution FTICR-MS analysis of fsTnT from human skeletal muscle biopsies.

Figure 4

(A) LC purified sTnT from human vastus lateralis muscle; (B) LC purified sTnT from human tibialis anterior muscle. Representative FTICR-MS spectra of human fsTnT, M40+ for fsTnT(III), M39+ for fsTnT(VI), M38+ for fsTnT(VII), respectively. Red circles represent theoretical isotopic abundance distribution of the isotopomer peaks. Solid circle(s) represents oxidation species. Star represents potential water loss (-18 Da). Calc’d, calculated most abundant mass; Expt’l, experimental most abundant mass. +98 represents the non-covalent phosphate adduct ((H3PO4, +98 Da). pfsTnT (III), pfsTnT (VI), and pfsTnT(VII) represents mono-phosphorylated fsTnT isoform III (P45378-3), isoform VI (P45378-6) and isoform, VII (P45378-7), respectively.