. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: J Muscle Res Cell Motil. 2015 Jan 23;36(2):169–181. doi: 10.1007/s10974-015-9404-6

Table 1. Human sTnI and sTnT proteoforms identified in Hytest Samples by high-resolution FTICR-MS.

Molecular weight (MW) of fsTnI was calculated based on DNA-predicted sequence of human fsTnI (UnitprotKB/Swiss-Prot P48788 (TNNI2_HUMAN)) with the removal of methionine and acetylation of the first amino acid at the N-terminus (+42 Da). The MW of ssTnI was calculated based on DNA-predicted sequence of human ssTnI (UnitprotKB/Swiss-Prot P19237 (TNNI1_HUMAN)) with removal of methionine at the N-terminus. The MW of ssTnT was calculated based on DNA-predicted sequence of human ssTnT (UnitprotKB/Swiss-Prot P13805-3) with the removal of N-terminal Met and acetylation at the new N-terminus.

Identification	Experimental MW	Calculated MW	Error (ppm)
Human sTn Isoforms
ssTnT(III)	31152.24	31152.07	5.46
fsTnI	21249.03	21248.90	6.12
ssTnI	21560.69	21560.55	6.49
Phosphorylation Products
_pssTnT(II)	30086.65	30086.51	4.65
_pssTnT(III)	31232.20	31232.04	5.12
Degradation Product
ssTnT(III) [P26-K262]	28270.16	28270.29	4.60