Figure 4. Quantitative PCR analysis of pro-inflammatory genes, up-regulated by eRNA in human monocytes.
Following treatment of monocytes with 10 µg/ml eRNA for 6 h, changes in cellular mRNA levels of representative pro-inflammatory mediators were quantified by RT-PCR. The dotted line indicates untreated controls. Data indicate changes in the ratio between target and β-actin mRNA expression relative to the levels in control cells under non-stimulatory conditions and represent mean ± SD from five independent experiments performed in triplicates; *p<0.05, #p<0.01.