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. 2015 May 5;10:3337–3350. doi: 10.2147/IJN.S77706

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© 2015 Chen et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Monolayer formation on different SF/P(LLA-CL) membranes. HCEC-B4G12 cells were cultured on different membranes for 1 week for monolayer formation.

Notes: Scanning electron microscopic images showed that the B4G12 cells could form a monolayer on all membranes and on glass coverslips (control group), as shown in (A). ZO-1 staining of monolayers is shown in (B). Scale bar, 20 μm.

Abbreviations: DAPI, 4,6-diamidino-2-phenylindole; SF, silk fibroin; P(LLA-CL), poly(l-lactic acid-co-ε-caprolactone); HCEC, human corneal endothelial cells; ZO-1, zona occludens-1.