Table 1.
Primers used for quantitative polymerase chain reaction
| Genes | Accession number | Forward (5′–3′) | Reverse (5′–3′) | Annealing temperature (°C) | Product size (base pairs) |
|---|---|---|---|---|---|
| AQP1 | NM_198098 | CTGGGCATCGAGATCATCGG | ATCCCACAGCCAGTGTAGTCA | 60 | 158 |
| Caspase-3 | NM_004346 | CATGGAAGCGAATCAATGGACT | CTGTACCAGACCGAGATGTCA | 60 | 139 |
| CLCN3 | NM_001243372 | GAGTTTTGCCTTTCTTGCAGTT | GAAAAGATATTTCCGCAGCAAC | 60 | 234 |
| Col-4 | NM_000092 | GCCGGTTGAATTTCCTTCATAT | ACCTTTAACGGCACCTAAAATGA | 60 | 232 |
| Col-8 | NM_001850 | GGAGATGCCCCACTTGCA | GCCGGTTGAATTTCCTTCATAT | 60 | 72 |
| CTNND1 | NM_001085465 | AGGATCCAGCAAACGATACAGT | AGGTCAGCTATGGCAGAAAGAG | 60 | 244 |
| GAPDH | NM_002046 | GAGATCCCTCCAAAATCAAGTG | GAGTCCTTCCACGATACCAAAG | 60 | 284 |
| IL-1 | NM_000576 | TGTACGATCACTGAACTGCA | GAAGTCAGTTATATCCTGGC | 60 | 433 |
| IL-8 | NM_000584 | TCTTGGCAGCCTTCCTGATT | TCCAGACAGAGCTCTCTTCCATC | 60 | 211 |
| Na+K+ATPase | NM_001160233 | ACGGCAGTGATCTAAAGGACAT | CAGAAGAATCATGTCAGCAGCTTG | 60 | 257 |
| SLC4A4 | NM_001098484 | GCTTGCAGATTACTACCCCATC | TTGAACACTCCTTCTTCGACAA | 60 | 209 |
| VDAC3 | NM_005662 | ATAAGTTGGCTGAAGGGTTGAA | TTCTGTGACAGTTTGGATTTGG | 60 | 235 |
| ZO-1 | NM_003257 | TTCTGAGGCCTGTAACCATTT | AATTGGATACCACTGGGCATAG | 60 | 245 |
Abbreviations: AQP1, aquaporin 1; caspase-3, apoptosis-related cysteine peptidase; CLCN3, voltage-sensitive 3 chloride channel; Col-4, collagen type IV; Col-8, collagen type VIII; CTNND1, cadherin-associated protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1, interleukin-1; IL-8, interleukin-8; Na+K+ATPase, Na+/K+ transporting, alpha 1 polypeptide; SLC4A4, sodium bicarbonate cotransporter member 4; VDAC3, voltage-dependent anion channel 3; ZO-1, zonula occludens-1.