Table 1.

List of substrates and proteins interacting with USP9X

Substrates	Major role of substrate	Interaction shown by	References
AF-6	Cell adhesion and polarity	Co-IP from brain lysates	[12, 109]
AGS3	Mitotic spindle orientation Golgi function	Tandem mass spectrometry, Co-IP from HEK293 and rat brain lysates	[27]
α-SYNUCLEIN	Synaptic maintenance Major component of Lewy bodies	Co-IP SH-SY5Y lysates and rat brain homogenates	[86]
ASK1	Apoptosis	Co-IP from HEK293A cells	[53]
BCL10	Activation of NFκB pathway	Co-IP JE6.1 cells	[110]
β-Catenin	Cell adhesion Wnt signalling	Co-IP from brain, L, MCF7 and T84 cells	[111]
EFA6	Cell adhesion and polarity	Co-IP from MDCK cell lysates	[23]
Epsin/liquid facets	Endocytosis Notch signalling	Co-IP from brain lysates, Drosophila eye disc protein extracts	[32, 35]
ErbB2	Oncogene	IP from SK-BR-3 cells	[10]
ERG	Transcription factor Prostate cancer	GST pulldowns and Co-IP from VCaP prostate cancer cells	[92]
ITCH	E3 Ligase	Co-IP from HEK293 and GST pulldown from brain lysates	[11, 37]
MARCH7	E3 Ligase Ubiquitin system	Sepharose pulldowns and IP from HEK293 cells	[112]
MARK4	Cell adhesion and polarity	Tandem affinity purification and Co-IP from HEK293 cells and brain lysates	[8]
MCL1	Apoptosis	Co-IP HEK293T cells	[24]
NUAK1	AMPK-related kinase	Tandem affinity purification and Co-IP from brain lysates and HEK293	[8]
PEX5	Peroxisomal protein shuttling	IP from rat liver and HeLa cell lysates	[7]
SMAD4	TGFβ signalling	Co-IP from HEK293	[9]
SMURF1	TGFβ/BMP signalling pathway	Quantitative mass spectrometry and Co-IP from HEK293 lysates	[65]
SMN	Maintenance of motor neurons	Mass spectrometry and Co-IP from HEK293 and HeLa cells	[42]
SURVIVIN	Mitosis Apoptosis	Co-IP from Xenopus oocytes and HeLa cells	[13]
VASA	Transcriptional regulation	Yeast two hybrid and tandem immunoprecipitation	[113]

Interactor	Major role of interactor	Interaction shown by	References
BAG1	Cell survival	Co-IP primary AML cells	[60]
DCX	Neuronal migration Trafficking	Co-IP from brain lysates	[82]
FOXO3	Transcription factor	IP from T74D breast cancer cell line	[114]
HDAC6	Transcriptional regulation	Mass spectrometry-based proteomics strategy	[115]
HUNTINGTIN	Trafficking Transcription	Yeast two-hybrid and Co-IP from brain	[88]
IKAP	Neurogenesis and cell migration	Mass spectrometry HEK293 cells	[116]
Imd	Innate immune response	Co-immunoprecipitation from S2 cells	[117]
Lebercilin	Leber congenital amaurosis	Quantitative protein complex analysis in HEK293 cells	[118]
Mind bomb1	E3 Ligase Signalling	GST pulldown from neuronal lysates, yeast two-hybrid screening and Co-IP	[39, 40]
p100	TNFa signalling	Tandem affinity purification	[119]
SALL4	Self-renewal	Mass spectrometry and protein interaction networks	[75]
SOX2	Transcription factor with critical roles in embryonic CNS development	Multi-dimensional protein identification technology in medulloblastoma cell line	[74]
TANK	Type I interferon induction	Mass spectrometry and protein interaction network	[120]
VMP1	Autophagy	Co-IP AR42J acinar cell lysates	[121]